Data frame of the effect of buffer compositions on DNA strand displacement amplification. A 4-d regression data set with with replication. This is a useful test data set for exercising function fitting methods.

Description

The BD data frame has 89 rows and 5 columns. There are 89 runs with four buffer components (KCL, MgCl2, KP04, dnTP) systematically varied in a space-filliing design. The response is the DNA amplification rate.

Usage

data(BD)

Format

This data frame contains the following columns:

KCl

Buffer component.

MgCl2

Buffer component.

KPO4

Buffer component.

dNTP

Buffer component, deoxyribonucleotides.

lnya

Exponential amplification rate on a log scale, i.e. the actual amplification rate.

Source

Thanks to Perry Haaland and Michael OConnell.

Becton Dickinson Research Center Research Triangle Park, NC

See Also

Tps

Examples

# fitting a DNA strand 
# displacement amplification  surface to various buffer compositions 
fit<- Tps(BD[,1:4],BD$lnya,scale.type="range") 
surface(fit)  # plots fitted surface and contours